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Zirchrom Columns to cope with extreem conditions

Zirconium and Titanium Columns 

Properties of Materials

Zirchrom Phase Separation ModeParticle diameter (um)Pore Diameter (A)Surface area (m2/g)Carbon load (%)pH RangeTemperature Limit (°C)
PBDReverse Phase3, 5300302.51 - 14150
PSReverse Phase3, 5300301.01 - 13150
EZReverse Phase3, 5300302.71 - 1050
MSReverse Phase3, 5300303.11 - 1080
SelectReverse Phase3, 530030-1 - 1080
CarbReverse Phase3, 530030-1 - 14150
DiamondBond C18Reverse Phase3, 5300303.51 - 14200
PhaseNormal Phase330030-1 - 14150
SAXStrong Anion Exchange3300301.21 - 1280
SHAXStrong Anion Exchange3300300.91 - 1280
WAXWeak Anion Exchange3300301.23 - 950
WCXWeak Cation Exchange330030-1 - 1050
PEZCation Exchange330030-1 - 1050
Sachtopore RP 13Reverse Phase330015-1 - 12100
Sachtopore NP 1Reverse Phase3, 5300151 - 14100

Zirchrom columns are used to create unique methods. Please contact us we like to assist you to create an innovative and winning solution.

Zirchrom columns can be divided into three modes of separation

Reverse Phase Mode

1. ZirChrom-PBD

Is produced by coating ultra-stable zirconia particles with an extremely thin layer of crosslinked polybutadiene. The chemical selectivity and efficiency of ZirChrom-PBD is similar to that of traditional C8 or C18 silica based columns for non-ionic analytes. However ZirChrom-PBD has greater stability than silica phases and higher efficiencies than polymer phases. Selectivity can be modified by the addition of a strong Lewis base, such as fluoride, phosphate or hydroxide, to the eluent. Figure 1 shows the high pH separation of ß-blockers using a LC-MS friendly amine buffer.

2. ZirChrom-EZ

Is a highly efficient phase created by first coating zirconia particles with an extremely thin layer of crosslinked polybutadiene and then deactivating the Lewis acid sites by applying a strong metal chelator. This enables Lewis base analytes such as carboxylates, sulphates and phosphates to be analysed throughout the pH range of 1 – 10 using conventional LC-MS buffers such as acetate or formate.

3. ZirChrom-MS

Is designed specifically for LC-MS, particularly applications involving basic pharmaceuticals. It has the same deactivation chemistry as ZirChrom-EZ but with a covalent attachment for low bleed and is approximately 2.5 times more retentive for simple compounds. The phase exhibits mixed mode retention characteristics – reversed-phase and cation-exchange.

4. ZirChrom-PS

Consists of zirconia coated with an extremely thin layer of polystyrene. The phase offers an alternative selectivity and less retention than ZirChrom-PBD making it ideal for non-polar analytes, or where highly aqueous eluents are necessary.

5. ZirChrom-SELECT

Is a lewis-acid site ‘deactivated’ carbon-coated zirconia phase. This deactivation reduces solute interactions with the zirconia surface, leading to improved peak shape and wider pharmaceutical compound applicability.

6. ZirChrom-CARB

Is produced by coating zirconia particles with a thin layer of elemental carbon. It is an alternative to porous graphitic carbon (PGC) supports for the separation of geometrical isomers, diastereomers and other closely related molecules. It offers very different selectivity to ODS or polymeric materials, making it an excellent choice for orthogonal screening in drug discovery.

6. DiamondBond-C18

Uses a proprietary covalent bonding technology to graft C18 groups to a carbon-clad zirconia, giving it outstanding stability for LC-MS applications. DiamondBond-C18 has selectivity that is intermediate between ODS silica and unbonded carbon supports for non-electrolytes.

 

Normal Phase Mode

1. ZirChrom PHASE

Ion Exchange Mode

1, ZirChrom-SAX

Is a highly efficient strong anion-exchanger, created by coating zirconia particles with a thin layer of crosslinked polyethyleneimine. It is useful for the separation of inorganic anions, organic anions and biomolecules such as nucleotides, nucleosides, amino acids and peptides.

2. ZirChrom-SHAX

Consists of quaternized polyethyleneiminecoated zirconia and is used for strong hydrophilic anion-exchange. It is more hydrophilic than ZirChrom-SAX, making it useful for the anion-exchange of proteins.

3. ZirChrom-WAX

Is a highly efficient weak anion-exchanger, produced by coating zirconia particles with a thin layer of crosslinked polyethyleneimine. In addition to separating inorganic anions, organic anions and biomolecules, it is an extremely stable amino phase for the separation of carbohydrates.

4. ZirChrom-WCX

Is a phosphate-coated zirconia phase for weak cation-exchange. It is useful for protein chromatography.

5. ZirChrom-PEZ

Is a EDTPA-coated zirconia phase for the cation-exchange chromatography of proteins and the separation of monoclonal antibodies.