Zirconium and Titanium Columns
Properties of Materials
|Zirchrom Phase||Separation Mode||Particle diameter (um)||Pore Diameter (A)||Surface area (m2/g)||Carbon load (%)||pH Range||Temperature Limit (°C)|
|PBD||Reverse Phase||3, 5||300||30||2.5||1 - 14||150|
|PS||Reverse Phase||3, 5||300||30||1.0||1 - 13||150|
|EZ||Reverse Phase||3, 5||300||30||2.7||1 - 10||50|
|MS||Reverse Phase||3, 5||300||30||3.1||1 - 10||80|
|Select||Reverse Phase||3, 5||300||30||-||1 - 10||80|
|Carb||Reverse Phase||3, 5||300||30||-||1 - 14||150|
|DiamondBond C18||Reverse Phase||3, 5||300||30||3.5||1 - 14||200|
|Phase||Normal Phase||3||300||30||-||1 - 14||150|
|SAX||Strong Anion Exchange||3||300||30||1.2||1 - 12||80|
|SHAX||Strong Anion Exchange||3||300||30||0.9||1 - 12||80|
|WAX||Weak Anion Exchange||3||300||30||1.2||3 - 9||50|
|WCX||Weak Cation Exchange||3||300||30||-||1 - 10||50|
|PEZ||Cation Exchange||3||300||30||-||1 - 10||50|
|Sachtopore RP 13||Reverse Phase||3||300||15||-||1 - 12||100|
|Sachtopore NP 1||Reverse Phase||3, 5||300||15||1 - 14||100|
Zirchrom columns are used to create unique methods. Please contact us we like to assist you to create an innovative and winning solution.
Zirchrom columns can be divided into three modes of separation
Reverse Phase Mode
Is produced by coating ultra-stable zirconia particles with an extremely thin layer of crosslinked polybutadiene. The chemical selectivity and efficiency of ZirChrom-PBD is similar to that of traditional C8 or C18 silica based columns for non-ionic analytes. However ZirChrom-PBD has greater stability than silica phases and higher efficiencies than polymer phases. Selectivity can be modified by the addition of a strong Lewis base, such as fluoride, phosphate or hydroxide, to the eluent. Figure 1 shows the high pH separation of ß-blockers using a LC-MS friendly amine buffer.
Is a highly efficient phase created by first coating zirconia particles with an extremely thin layer of crosslinked polybutadiene and then deactivating the Lewis acid sites by applying a strong metal chelator. This enables Lewis base analytes such as carboxylates, sulphates and phosphates to be analysed throughout the pH range of 1 – 10 using conventional LC-MS buffers such as acetate or formate.
Is designed specifically for LC-MS, particularly applications involving basic pharmaceuticals. It has the same deactivation chemistry as ZirChrom-EZ but with a covalent attachment for low bleed and is approximately 2.5 times more retentive for simple compounds. The phase exhibits mixed mode retention characteristics – reversed-phase and cation-exchange.
Consists of zirconia coated with an extremely thin layer of polystyrene. The phase offers an alternative selectivity and less retention than ZirChrom-PBD making it ideal for non-polar analytes, or where highly aqueous eluents are necessary.
Is a lewis-acid site ‘deactivated’ carbon-coated zirconia phase. This deactivation reduces solute interactions with the zirconia surface, leading to improved peak shape and wider pharmaceutical compound applicability.
Is produced by coating zirconia particles with a thin layer of elemental carbon. It is an alternative to porous graphitic carbon (PGC) supports for the separation of geometrical isomers, diastereomers and other closely related molecules. It offers very different selectivity to ODS or polymeric materials, making it an excellent choice for orthogonal screening in drug discovery.
Uses a proprietary covalent bonding technology to graft C18 groups to a carbon-clad zirconia, giving it outstanding stability for LC-MS applications. DiamondBond-C18 has selectivity that is intermediate between ODS silica and unbonded carbon supports for non-electrolytes.
Normal Phase Mode
1. ZirChrom PHASE
Ion Exchange Mode
Is a highly efficient strong anion-exchanger, created by coating zirconia particles with a thin layer of crosslinked polyethyleneimine. It is useful for the separation of inorganic anions, organic anions and biomolecules such as nucleotides, nucleosides, amino acids and peptides.
Consists of quaternized polyethyleneiminecoated zirconia and is used for strong hydrophilic anion-exchange. It is more hydrophilic than ZirChrom-SAX, making it useful for the anion-exchange of proteins.
Is a highly efficient weak anion-exchanger, produced by coating zirconia particles with a thin layer of crosslinked polyethyleneimine. In addition to separating inorganic anions, organic anions and biomolecules, it is an extremely stable amino phase for the separation of carbohydrates.
Is a phosphate-coated zirconia phase for weak cation-exchange. It is useful for protein chromatography.
Is a EDTPA-coated zirconia phase for the cation-exchange chromatography of proteins and the separation of monoclonal antibodies.